بررسی اثر عصاره آبی و آبی‌الکلی دانه زیره سیاه (Bunium persicum) بر میزان ترشح انسولین از جزایر لانگرهانس جداشده موش سوری نر

 Background & Aims: As B. persicum is used in treatment of diabetes, we aimed to study the effect of B. persicum seeds extract on level of insulin secretion from isolated islets of langerhans in male mouse.Materials & Methods: In this experimental study, adult male mice (NMARI) weighting 20-25g were purchased. Islets of langerhans were isolated by collagenase digestion method and were divided into 18 groups: (glucose2.8mM and 16.7mM), aqueous and hydro-alcoholic extracts of B. persicum seeds (0.05, 0.1, 1mg/ml) and glyburide (1, 10μM). Each concentration of extracts and glyburide applied separately on isolated islets. Insulin secretion from isolated islets was evaluated by a static incubation system, and insulin secretion amount was measured by IRMA method.Results: Amount of insulin secretion in 0.05, 0.1mg/ml concentrations of B. persicum seed extracts in mediums containing glucose2.8 mM and 16.7 mM increased significantly in comparison with glucose2.8 mM and 16.7 mM groups. 0.05mg/ml of aqueous extract in mediums containing glucose 2.8 mM and 16.7 mM increased insulin secretion when compared to various concentrations of hydro-alcoholic extracts. also among all groups, glyburide10μM showed most effective in increasing insulin secretion from isolated islets in comparison with glucose2.8mM and 16.7mM groups (p<0.001).Conclusion: Overall, the results showed, seed’s extract of B. persicum in low concentrations was able to increase insulin secretion and this effect is similar to the effect of glyburide1μM on the secretion of this hormone in glucose2.8mM medium as well as glyburide10μM in glucose16.7mM medium. So it’s suggested that one of the B. persicum ablative blood sugar mechanisms is via the effect on langerhans islets and stimulation of insulin secretion. SOURCE: URMIA MED J 2014: 25(8): 751 ISSN: 1027-3727